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Molecular Cloning
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Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
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Внешние ссылки главной страницы ( 4 ) | |
cshlpress.com/checkout.tpl?sku=934&action=mc4&typ2=mc4dotcom | <img> |
laskerfoundation.org/awards/2012_s_description.htm | Tom Maniatis wins Lasker |
occamstypewriter.org/trading-knowledge/2012/09/14/not-quite-... | 'Not Quite a Book Prize' |
cshlpress.org | © 2012 CSHL Press |
Внутренние ссылки главной страницы ( 248 ) | |
/index.php | Home |
index.php?prt=1 | Protocol 1: Preparation of Plasmid DNA by Alkaline Lysis with SDS: Minipreps |
index.php?prt=2 | Protocol 2: Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreps |
index.php?prt=3 | ) |
index.php?prt=4 | Protocol 4: Precipitation of DNA with Ethanol |
index.php?prt=5 | Protocol 5: Precipitation of DNA with Isopropanol |
index.php?prt=6 | Protocol 6: Concentrating and Desalting Nucleic Acids with Microconcentrators |
index.php?prt=7 | Protocol 7: Concentrating Nucleic Acids by Extraction with Butanol |
index.php?prt=8 | Protocol 8: Preparation of Single-Stranded Bacteriophage M13 DNA by Precipitation with Polyethylene Glycol |
index.php?prt=9 | Protocol 9: Plating Bacteriophage M13 |
index.php?prt=10 | Protocol 10: Growing Bacteriophage M13 in Liquid Culture |
index.php?prt=11 | Protocol 11: Preparation of Double-Stranded (Replicative Form) Bacteriophage M13 DNA |
index.php?prt=12 | Protocol 12: Isolation of High-Molecular-Weight DNA Using Organic Solvents to Purify DNA |
index.php?prt=13 | Protocol 13: Isolation of High-Molecular-Weight DNA from Mammalian Cells Using Proteinase K and Phenol |
index.php?prt=14 | Protocol 14: A Single-Step Method for the Simultaneous Preparation of DNA, RNA, and Protein from Cells and Tissues |
index.php?prt=15 | Protocol 15: Preparation of Genomic DNA from Mouse Tails and Other Small Samples |
index.php?prt=16 | Protocol 16: Rapid Isolation of Yeast DNA |
index.php?prt=17 | Protocol 17: Using Ethidium Bromide to Estimate the Amount of DNA in Bands after Electrophoresis through Minigels |
index.php?prt=18 | Protocol 18: Estimating the Concentration of DNA by Fluorometry Using Hoechst 33258 |
index.php?prt=19 | Protocol 19: Quantifying DNA in Solution with PicoGreen |
index.php?prt=20 | Protocol 1: Agarose Gel Electrophoresis |
index.php?prt=21 | Protocol 2: Detection of DNA in Agarose Gels by Staining |
index.php?prt=22 | Protocol 3: Polyacrylamide Gel Electrophoresis |
index.php?prt=23 | Protocol 4: Detection of DNA in Polyacrylamide Gels by Staining |
index.php?prt=24 | Protocol 5: Detection of DNA in Polyacrylamide Gels by Autoradiography |
index.php?prt=25 | Protocol 6: Alkaline Agarose Gel Electrophoresis |
index.php?prt=26 | Protocol 7: Imaging: Autoradiography and Phosphorimaging |
index.php?prt=27 | Protocol 8: Recovery of DNA from Agarose Gels Using Glass Beads |
index.php?prt=28 | Protocol 9: Recovery of DNA from Low-Melting-Temperature Agarose Gels: Organic Extraction |
index.php?prt=29 | Protocol 10: Isolation of DNA Fragments from Polyacrylamide Gels by the Crush and Soak Method |
index.php?prt=30 | Protocol 11: Southern Blotting |
index.php?prt=31 | Protocol 12: Southern Blotting: Simultaneous Transfer of DNA from an Agarose Gel to Two Membranes |
index.php?prt=32 | Protocol 13: Southern Hybridization of Radiolabeled Probes to Nucleic Acids Immobilized on Membranes |
index.php?prt=33 | : High-Efficiency Transformation |
index.php?prt=34 | : Ultracompetent Cells |
index.php?prt=35 | : Nanoparticle-Mediated Transformation |
index.php?prt=36 | by Electroporation |
index.php?prt=37 | Protocol 5: Cloning in Plasmid Vectors: Directional Cloning |
index.php?prt=38 | Protocol 6: Cloning in Plasmid Vectors: Blunt-End Cloning |
index.php?prt=39 | Protocol 7: Dephosphorylation of Plasmid DNA |
index.php?prt=40 | Protocol 8: Attaching Phosphorylated Adaptors/Linkers to Blunt-Ended DNAs |
index.php?prt=41 | Protocol 9: Cloning PCR Products: Addition of Restriction Sites to the Termini of Amplified DNA |
index.php?prt=42 | Protocol 10: Cloning PCR Products: Blunt-End Cloning |
index.php?prt=43 | Protocol 11: Cloning PCR Products: Making T Vectors |
index.php?prt=44 | Protocol 12: Cloning PCR Products: TA Cloning |
index.php?prt=45 | Protocol 13: Cloning PCR Products: TOPO TA Cloning |
index.php?prt=46 | Protocol 14: Screening Bacterial Colonies Using X-Gal and IPTG: -Complementation |
index.php?prt=47 | Protocol 1: Propagating Gateway Vectors |
index.php?prt=48 | Protocol 2: Generating an ORF Entry Clone and Destination Clone |
index.php?prt=49 | Protocol 3: Using Multisite LR Cloning to Generate a Destination Clone |
index.php?prt=50 | Protocol 1: Small-Scale Isolation of BAC DNA and Verification by PCR |
index.php?prt=51 | Protocol 2: Large-Scale Preparation and Linearization of BAC DNA |
index.php?prt=52 | Protocol 3: Examination of BAC DNA Quality and Quantity by Pulsed-Field Gel Electrophoresis |
index.php?prt=53 | Protocol 4: Two-Step BAC Engineering: Preparation of Shuttle Vector DNA |
index.php?prt=54 | Protocol 5: Preparation of the A Homology Arm (A-Box) and B Homology Arm (B-Box) |
index.php?prt=55 | Protocol 6: Cloning of the A and B Homology Arms into the Shuttle Vector |
index.php?prt=56 | Protocol 7: Preparation and Verification of the Recombinant Shuttle Vector |
index.php?prt=57 | Protocol 8: Electroporation of Competent BAC Host Cells with the Recombinant Shuttle Vector |
index.php?prt=58 | Protocol 9: Verification of Cointegrates and Selection of Resolved BAC Clones |
index.php?prt=59 | Protocol 10: One-Step BAC Modification: Preparation of Plasmids |
index.php?prt=60 | Protocol 11: Preparation of the A Homology Arm (A-Box) |
index.php?prt=61 | Protocol 12: Cloning of the A Homology Arm into Reporter-Shuttle Vector |
index.php?prt=62 | Protocol 13: Transformation of the BAC Host with the RecA Vector |
index.php?prt=63 | Protocol 14: Transfer of the Reporter Vector into BAC/RecA Cells and Selection of Cointegrates |
index.php?prt=64 | and Preparation of DNA |
index.php?prt=65 | Protocol 16: Small-Scale Preparations of Yeast DNA |
index.php?prt=66 | Protocol 1: Purification of Total RNA from Mammalian Cells and Tissues |
index.php?prt=67 | Protocol 2: Isolation of Total RNA from Zebrafish Embryos and Adults |
index.php?prt=68 | Protocol 3: Total RNA Isolation from Drosophila melanogaster |
index.php?prt=69 | Protocol 4: Total RNA Extraction from Caenorhabditis elegans |
index.php?prt=70 | Using Hot Acid Phenol |
index.php?prt=71 | Protocol 6: Quantifying and Storing RNA |
index.php?prt=72 | Protocol 7: Precipitation of RNA with Ethanol |
index.php?prt=73 | Protocol 8: Removing DNA Contamination from RNA Samples by Treatment with RNase-Free DNase I |
index.php?prt=74 | Messenger RNA Using Magnetic Oligo(dT) Beads |
index.php?prt=75 | Protocol 10: Separation of RNA according to Size: Electrophoresis of RNA through Agarose Gels Containing Formaldehyde |
index.php?prt=76 | Protocol 11: Separation of RNA according to Size: Electrophoresis of RNA through Denaturing Urea Polyacrylamide Gels |
index.php?prt=77 | Protocol 12: Transfer and Fixation of Denatured RNA in Agarose Gels to Membranes |
index.php?prt=78 | Protocol 13: Transfer and Fixation of Denatured RNA in Polyacrylamide Gels to Membranes by Electrophoretic Transfer |
index.php?prt=79 | Protocol 14: Northern Hybridization |
index.php?prt=80 | Protocol 15: Dot and Slot Hybridization of Purified RNA |
index.php?prt=81 | Protocol 16: Mapping RNA with Nuclease S1 |
index.php?prt=82 | Protocol 17: Ribonuclease Protection: Mapping RNA with Ribonuclease and Radiolabeled RNA Probes |
index.php?prt=83 | Protocol 18: Analysis of RNA by Primer Extension |
index.php?prt=84 | Protocol 1: The Basic Polymerase Chain Reaction |
index.php?prt=85 | Protocol 2: Hot Start PCR |
index.php?prt=86 | Protocol 3: Touchdown PCR |
index.php?prt=87 | Protocol 4: PCR Amplification of GC-Rich Templates |
index.php?prt=88 | Protocol 5: Long and Accurate PCR (LA PCR) |
index.php?prt=89 | Protocol 6: Inverse PCR |
index.php?prt=90 | Protocol 7: Nested PCR |
index.php?prt=91 | Protocol 8: Amplification of cDNA Generated by Reverse Transcription of mRNA: Two-Step RT-PCR |
index.php?prt=92 | Protocol 9: Rapid Amplification of Sequences from the 5 Ends of mRNAs: 5-RACE |
index.php?prt=93 | Protocol 10: Rapid Amplification of Sequences from the 3 Ends of mRNAs: 3-RACE |
index.php?prt=94 | Protocol 11: Screening Colonies by PCR |
index.php?prt=95 | Protocol 1: Visualizing Genomic Annotations with the UCSC Genome Browser |
index.php?prt=96 | Protocol 2: Sequence Alignment and Homology Search with BLAST and ClustalW |
index.php?prt=97 | Protocol 3: Designing PCR Primers Using Primer3Plus |
index.php?prt=98 | Protocol 4: Expression Profiling by Microarray and RNA-seq |
index.php?prt=99 | Protocol 5: Mapping Billions of Short Reads to a Reference Genome |
index.php?prt=100 | Protocol 6: Identifying Regions Enriched in a ChIP-seq Data Set (Peak Finding) |
index.php?prt=101 | -Regulatory Motifs |
index.php?prt=102 | Protocol 1: Optimizing Primer and Probe Concentrations for Use in Real-Time PCR |
index.php?prt=103 | Protocol 2: Constructing a Standard Curve |
index.php?prt=104 | Protocol 3: Quantification of DNA by Real-Time PCR |
index.php?prt=105 | Protocol 4: Quantification of RNA by Real-Time RT-PCR |
index.php?prt=106 | Protocol 5: Analysis and Normalization of Real-Time PCR Experimental Data |
index.php?prt=107 | Click here for free access to Chapter 10 in its entirety |
index.php?prt=108 | Protocol 2: Round A/Round B Amplification of DNA |
index.php?prt=109 | Protocol 3: T7 Linear Amplification of DNA (TLAD) for Nucleosomal and Other DNA < 500 bp |
index.php?prt=110 | Protocol 4: Amplification of RNA |
index.php?prt=111 | Protocol 5: Direct Cyanine-dUTP Labeling of RNA |
index.php?prt=112 | Protocol 6: Indirect Aminoallyl-dUTP Labeling of RNA |
index.php?prt=113 | Protocol 7: Cyanine-dCTP Labeling of DNA Using Klenow |
index.php?prt=114 | Protocol 8: Indirect Labeling of DNA |
index.php?prt=115 | Protocol 9: Blocking Polylysines on Homemade Microarrays |
index.php?prt=116 | Protocol 10: Hybridization to Homemade Microarrays |
index.php?prt=117 | Protocol 1: Preparing Plasmid Subclones for Capillary Sequencing |
index.php?prt=118 | Protocol 2: Preparing PCR Products for Capillary Sequencing |
index.php?prt=119 | Protocol 3: Cycle-Sequencing Reactions |
index.php?prt=120 | Protocol 4: Whole Genome: Manual Library Preparation |
index.php?prt=121 | Protocol 5: Whole Genome: Automated, Nonindexed Library Preparation |
index.php?prt=122 | Protocol 6: Whole Genome: Automated, Indexed Library Preparation |
index.php?prt=123 | Protocol 7: Preparation of a 3-kb Mate-Pair Library for Illumina Sequencing |
index.php?prt=124 | Protocol 8: Preparation of an 8-kb Mate-Pair Library for Illumina Sequencing |
index.php?prt=125 | Protocol 9: RNA-Seq: RNA Conversion to cDNA and Amplification |
index.php?prt=126 | Protocol 10: Solution-Phase Exome Capture |
index.php?prt=127 | Protocol 11: Automated Size Selection |
index.php?prt=128 | Protocol 12: Library Quantification Using SYBR Green-qPCR |
index.php?prt=129 | Protocol 13: Library Quantification Using PicoGreen Fluorometry |
index.php?prt=130 | Protocol 14: Library Quantification: Fluorometric Quantitation of Double-Stranded or Single-Stranded DNA Samples Using the Qubit... |
index.php?prt=131 | Protocol 15: Preparation of Small-Fragment Libraries for 454 Sequencing |
index.php?prt=132 | Protocol 16: sstDNA Library Capture and emPCR |
index.php?prt=133 | Protocol 17: Roche/454 Sequencer: Executing a Sequencing Run |
index.php?prt=134 | Protocol 18: Validation |
index.php?prt=135 | Protocol 19: Quality Assessment of Sequence Data |
index.php?prt=136 | Protocol 20: Data Analysis |
index.php?prt=137 | Protocol 1: DNA Bisulfite Sequencing for Single-Nucleotide-Resolution DNA Methylation Detection |
index.php?prt=138 | Protocol 2: Methylation-Specific PCR for Gene-Specific DNA Methylation Detection |
index.php?prt=139 | Protocol 3: Methyl-Cytosine-Based Immunoprecipitation for DNA Methylation Analysis |
index.php?prt=140 | Protocol 4: High-Throughput Deep Sequencing for Mapping Mammalian DNA Methylation |
index.php?prt=141 | Protocol 5: Roche 454 Clonal Sequencing of Bisulfite-Converted DNA Libraries |
index.php?prt=142 | Protocol 6: Illumina Sequencing of Bisulfite-Converted DNA Libraries |
index.php?prt=143 | Protocol 1: Random Priming: Labeling of Purified DNA Fragments by Extension of Random Oligonucleotides |
index.php?prt=144 | Protocol 2: Random Priming: Labeling of DNA by Extension of Random Oligonucleotides in the Presence of Melted Agarose |
index.php?prt=145 | Protocol 3: Labeling of DNA Probes by Nick Translation |
index.php?prt=146 | Protocol 4: Labeling of DNA Probes by Polymerase Chain Reaction |
index.php?prt=147 | Protocol 5: Synthesis of Single-Stranded RNA Probes by In Vitro Transcription |
index.php?prt=148 | Protocol 6: Synthesis of cDNA Probes from mRNA Using Random Oligonucleotide Primers |
index.php?prt=149 | Protocol 7: Radiolabeling of Subtracted cDNA Probes by Random Oligonucleotide Extension |
index.php?prt=150 | DNA Polymerase I |
index.php?prt=151 | Protocol 9: Dephosphorylation of DNA Fragments with Alkaline Phosphatase |
index.php?prt=152 | Protocol 10: Phosphorylation of DNA Molecules with Protruding 5-Hydroxyl Termini |
index.php?prt=153 | Protocol 11: Phosphorylation of DNA Molecules with Dephosphorylated Blunt Ends or Recessed 5 Termini |
index.php?prt=154 | Protocol 12: Phosphorylating the 5 Termini of Oligonucleotides Using T4 Polynucleotide Kinase |
index.php?prt=155 | Protocol 13: Labeling the 3 Termini of Oligonucleotides Using Terminal Deoxynucleotidyl Transferase |
index.php?prt=156 | DNA Polymerase I |
index.php?prt=157 | Protocol 15: Purification of Labeled Oligonucleotides by Precipitation with Ethanol |
index.php?prt=158 | Protocol 16: Purification of Labeled Oligonucleotides by Size-Exclusion Chromatography |
index.php?prt=159 | Column |
index.php?prt=160 | Protocol 18: Hybridization of Oligonucleotide Probes in Aqueous Solutions: Washing in Buffers Containing Quaternary Ammonium Sal... |
index.php?prt=161 | Protocol 1: Random Mutagenesis Using Error-Prone DNA Polymerases |
index.php?prt=162 | Protocol 2: Creating Insertions or Deletions Using Overlap Extension PCR Mutagenesis |
index.php?prt=163 | Protocol 3: In Vitro Mutagenesis Using Double-Stranded DNA Templates: Selection of Mutants with DpnI |
index.php?prt=164 | Protocol 4: Altered -Lactamase Selection Approach for Site-Directed Mutagenesis |
index.php?prt=165 | Protocol 5: Oligonucleotide-Directed Mutagenesis by Elimination of a Unique Restriction Site (USE Mutagenesis) |
index.php?prt=166 | Protocol 6: Saturation Mutagenesis by Codon Cassette Insertion |
index.php?prt=167 | Protocol 7: Random Scanning Mutagenesis |
index.php?prt=168 | Protocol 8: Multisite-Directed Mutagenesis |
index.php?prt=169 | Protocol 9: Megaprimer PCR-Based Mutagenesis |
index.php?prt=170 | Protocol 1: DNA Transfection Mediated by Cationic Lipid Reagents |
index.php?prt=171 | Protocol 2: Calcium-Phosphate-Mediated Transfection of Eukaryotic Cells with Plasmid DNAs |
index.php?prt=172 | Protocol 3: Calcium-Phosphate-Mediated Transfection of Cells with High-Molecular-Weight Genomic DNA |
index.php?prt=173 | Protocol 4: Transfection Mediated by DEAE-Dextran: High-Efficiency Method |
index.php?prt=174 | Protocol 5: DNA Transfection by Electroporation |
index.php?prt=175 | Protocol 6: Analysis of Cell Viability by the alamarBlue Assay |
index.php?prt=176 | Protocol 7: Analysis of Cell Viability by the Lactate Dehydrogenase Assay |
index.php?prt=177 | Protocol 8: Analysis of Cell Viability by the MTT Assay |
index.php?prt=178 | Protocol 1: Construction of Recombinant Adenovirus Genomes by Direct Cloning |
index.php?prt=179 | Protocol 2: Release of the Cloned Recombinant Adenovirus Genome for Rescue and Expansion |
index.php?prt=180 | Protocol 3: Purification of the Recombinant Adenovirus by Cesium Chloride Gradient Centrifugation |
index.php?prt=181 | Protocol 4: Characterization of the Purified Recombinant Adenovirus for Viral Genome Structure by Restriction Enzyme Digestions |
index.php?prt=182 | End-Point Dilution and qPCR |
index.php?prt=183 | Protocol 6: Detection Assay for Replication-Competent Adenovirus by Concentration Passage and Real-Time qPCR |
index.php?prt=184 | Protocol 7: Production of rAAVs by Transient Transfection |
index.php?prt=185 | Protocol 8: Purification of rAAVs by Cesium Chloride Gradient Sedimentation |
index.php?prt=186 | Protocol 9: Purification of rAAVs by Iodixanol Gradient Centrifugation |
index.php?prt=187 | Protocol 10: Purification of rAAV2s by Heparin Column Affinity Chromatography |
index.php?prt=188 | Protocol 11: Enrichment of Fully Packaged Virions in Column-Purified rAAV Preparations by Iodixanol Gradient Centrifugation Foll... |
index.php?prt=189 | Protocol 12: Titration of rAAV Genome Copy Number Using Real-Time qPCR |
index.php?prt=190 | End-Point Dilution and qPCR |
index.php?prt=191 | Protocol 14: Analysis of rAAV Sample Morphology Using Negative Staining and High-Resolution Electron Microscopy |
index.php?prt=192 | Protocol 15: Analysis of rAAV Purity Using Silver-Stained SDS-PAGE |
index.php?prt=193 | Protocol 16: Production of High-Titer Retrovirus and Lentivirus Vectors |
index.php?prt=194 | Protocol 17: Titration of Lentivirus Vectors |
index.php?prt=195 | Protocol 18: Monitoring Lentivirus Vector Stocks for Replication-Competent Viruses |
index.php?prt=196 | Protocol 1: Assay for -Galactosidase in Extracts of Mammalian Cells |
index.php?prt=197 | Protocol 2: Single Luciferase Reporter Assay |
index.php?prt=198 | Protocol 3: Dual Luciferase Reporter Assay |
index.php?prt=199 | Protocol 4: Using ELISA to Measure GFP Production |
index.php?prt=200 | Protocol 5: Generation of Cell Lines with Tetracycline-Regulated Gene Expression |
index.php?prt=201 | Protocol 1: Preparation of siRNA Duplexes |
index.php?prt=202 | Protocol 2: RNAi in Mammalian Cells by siRNA Duplex Transfection |
index.php?prt=203 | S2 Cells by siRNA Duplex Transfection |
index.php?prt=204 | Protocol 4: Preparation of dsRNAs by In Vitro Transcription |
index.php?prt=205 | S2 Cells by dsRNA Soaking |
index.php?prt=206 | S2 Cells by dsRNA Transfection |
index.php?prt=207 | Protocol 7: Analysis of Small RNAs by Northern Hybridization |
index.php?prt=208 | Protocol 8: Analysis of Small RNAs by Quantitative Reverse Transcription PCR |
index.php?prt=209 | Protocol 9: Construction of Small RNA Libraries for High-Throughput Sequencing |
index.php?prt=210 | Protocol 10: Preparation of Antisense Oligonucleotides to Inhibit miRNA Function |
index.php?prt=211 | Protocol 11: Inhibiting miRNA Function by Antisense Oligonucleotides in Cultured Mammalian Cells |
index.php?prt=212 | S2 Cells |
index.php?prt=213 | Using IPTG-Inducible Promoters |
index.php?prt=214 | Protocol 2: Expression of Cloned Genes Using the Baculovirus Expression System |
index.php?prt=215 | Using the Methanol-Inducible Promoter AOX1 |
index.php?prt=216 | Protocol 4: Preparation of Cell Extract for Purification of Soluble Proteins Expressed in E. coli |
index.php?prt=217 | Protocol 5: Purification of Polyhistidine-Tagged Proteins by Immobilized Metal Affinity Chromatography |
index.php?prt=218 | Protocol 6: Purification of Fusion Proteins by Affinity Chromatography on Glutathione Resin |
index.php?prt=219 | Protocol 7: Solubilization of Expressed Proteins from Inclusion Bodies |
index.php?prt=220 | Protocol 8: SDS-PAGE of Proteins |
index.php?prt=221 | Protocol 9: Analysis of Proteins by Immunoblotting |
index.php?prt=222 | Protocol 10: Methods for Measuring the Concentrations of Proteins |
index.php?prt=223 | Protocol 1: Formaldehyde Cross-Linking |
index.php?prt=224 | Protocol 2: Preparation of Cross-Linked Chromatin for ChIP |
index.php?prt=225 | Protocol 3: ChIP |
index.php?prt=226 | Protocol 4: ChIPQuantitative PCR (ChIP-qPCR) |
index.php?prt=227 | Protocol 5: ChIP-chip |
index.php?prt=228 | Protocol 6: ChIP-seq |
index.php?prt=229 | Protocol 7: Generation of 3C Libraries from Cross-Linked Cells |
index.php?prt=230 | Protocol 8: Generation of ChIP-loop Libraries |
index.php?prt=231 | Protocol 9: Generation of Control Ligation Product Libraries |
index.php?prt=232 | Protocol 10: PCR Detection of 3C Ligation Products Present in 3C, ChIP-loop, and Control Libraries: Library Titration and Intera... |
index.php?prt=233 | Protocol 11: 4C Analysis of 3C, ChIP-loop, and Control Libraries |
index.php?prt=234 | Protocol 12: 5C Analysis of 3C, ChIP-loop, and Control Libraries |
index.php?prt=235 | Protocol 1: Optimization of Immunoprecipitation Stringency for CLIP |
index.php?prt=236 | Protocol 2: UV Cross-Linking of Live Cells and Lysate Preparation |
index.php?prt=237 | Protocol 3: RNase Titration, Immunoprecipitation, and SDS-PAGE |
index.php?prt=238 | Protocol 4: 3-Linker Ligation and Size Selection by SDS-PAGE |
index.php?prt=239 | Protocol 5: Isolation of the RNA Tags, 5-Linker Ligation, and Reverse Transcription PCR Amplification |
index.php?prt=240 | Protocol 6: Sequencing of RNA CLIP Tags |
index.php?prt=241 | Protocol 7: Gel Purification and Storage of RNA Linkers |
index.php?prt=242 | Protocol 1: Generating Yeast One-Hybrid DNA-Bait Strains |
index.php?prt=243 | Protocol 2: Generating Yeast Two-Hybrid Bait Strains |
index.php?prt=244 | Protocol 3: Identifying Interactors from an Activation Domain Prey Library |
index.php?prt=245 | Protocol 4: High-Efficiency Yeast Transformation |
index.php?prt=246 | Protocol 5: Colony Lift Colorimetric Assay for -Galactosidase Activity |
index.php?prt=247 | Protocol 6: Yeast Colony PCR |
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URL of the ICANN Whois Inaccuracy Complaint Form: https://www.icann.org/wicf/
>>> Last update of whois database: 2018-10-19T14:45:28Z <<<
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The Registry database contains ONLY .COM, .NET, .EDU domains and
Registrars.
ФРГ - Деттинген-унтер-Тек - 176.9.118.138
ФРГ - Деттинген-унтер-Тек - 176.9.118.139
Нидерланды - Амстердам - 85.17.177.246
Hetzner Online GmbH
LeaseWeb
Hetzner Online GmbH
LeaseWeb B.V.
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